ABSTRACT
Background: Obesity and overweight is a disorder of unusually increased body fat generally resulting from increased energy intake relative to energy expenditure and is a primary sustenance-related disorder globally. The extensive increase in its prevalence in current years and its association with reduced life expectancy has made obesity one of the most vital public health problems. The purpose of the study was to determine the relationship between serum lipid profile and blood pressure with body mass index (BMI). Material & Methods: A cross-sectional study was carried out in the Department of Physiology and Biochemistry, Rangpur Medical College, Rangpur from January 2013 to December 2013. A purposive sampling technique was followed. A total number of 90 people from 18 to 45 years old were included in the study, categorized into three groups, such as Group-A:(Control 30): Healthy subject of normal weight, Group-B:(Experimental 30): Healthy subject of overweight & Group-C(Experimental-30): Healthy subjects of obese. Verbal consent was taken before recruiting the study population. Completed data forms were reviewed, edited, and processed for computer data entry. The data analysis was performed using the “t” test, “r” test & Statistical Package for the Social Sciences (SPSS) Version 25.0. Results: In group A, the mean BMI of patients was 18.5-22.9, in group B mean BMI of patients was 23.0-24.9, and in group C, the mean BMI of people was 25.0 or greater. The mean ± SD serum LDL-C levels were 107.77 ± 26.720 mg/dl in group A and 134.70 ± 41.787 mg/dl in group B. There was a significant difference (p<0.001) between the two groups. The mean ± SD pulse pressure levels were 38 ± 6.644 mmHg in group A and 41.67 ± 11.167 mmHg in group B. There was no significant difference (p>0.05) between the two groups. Serum total cholesterol levels were positively correlated in groups A & B but the relationship of serum total cholesterol levels was statistically significant in groups A and B. Blood pressure levels were positively correlated in groups A &C but the relationship was statistically non-significant. Conclusion: In this current content, it is difficult to define the specific mechanism involved for significantly higher serum total cholesterol, serum triglyceride, and serum LDL-C levels and non-significantly lesser serum HDL-C levels in overweight & obese people and also non-significantly higher blood pressure in overweight people but significantly higher blood pressure in obese subjects.
ABSTRACT
Crude methanolic extract and other fractions of Sterculia villosa barks were investigated for their antimicrobial, cytotoxic and antioxidant activity in this study. Antimicrobial activity of different extract was evaluated by measuring the diameter of the zone of inhibition against gram-positive & gram-negative bacteria and fungi using Ciprofloxacin as a standard antimicrobial agent. Free radical scavenging activity for the same extracts was determined by DPPH assay where BHT was used as positive control and Cytotoxicity was determined by Brine Shrimp nauplii where the minimum inhibitory concentration was assessed by serial dilution technique. Mild antimicrobial activity was found; crude methanolic extract showed effect against all the organisms, while other extracts showed effect for some of the organisms. The LC50 value for cytotoxicity assay was found 0.3, 2.95, 3.76, 35.33 & 55.98 μg/ml for CSV, PESV, CTSV, DCMSV & EASV extracts respectively where LC50 value of Vincristine Sulfate was 0.544 μg/ml. Ethyl acetate fraction showed good antioxidant properties and except Pet Ether fraction all other extracts showed considerable antioxidant activity. The bark of Sterculia villosa can be considered for further research for finding potent compounds of antioxidant, antimicrobial and cytotoxic activity.